basic fibroblast growth factor (R&D Systems)
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R&D Systems
basic fibroblast growth factor
Basic Fibroblast Growth Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 890 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+basic+fgf/Recombinant+Human+FGF+basic%2FFGF2%2FbFGF+(146+aa)+Protein/pmc13132546-182-33-37
Average 96 stars, based on 890 article reviews
Basic Fibroblast Growth Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 890 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+basic+fgf/Recombinant+Human+FGF+basic%2FFGF2%2FbFGF+(146+aa)+Protein/pmc13132546-182-33-37
Average 96 stars, based on 890 article reviews
basic fibroblast growth factor - by Bioz Stars,
2026-09
96/100 stars
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Cell Culture:Article Title: p53 Inhibits Bmi-1-driven Self-Renewal and Defines Salivary Gland Cancer Stemness Article Snippet: .. UM-HMC-1,-3A,-3B (RRID:CVCL_Y473, RRID:CVCL_Y471, RRID:CVCL_Y472, respectively) cells (23) were cultured in ultralow attachment plates or flasks (Corning) in DMEM/F12 (Invitrogen) supplemented with 1% N2 Supplement (Invitrogen), 1% GlutaMAX (Invitrogen), 1% antibiotic–antimycotic (Millipore Sigma), 20 ng/mL rhEGF (R&D Systems), 20 ng/mL Recombinant:Article Title: p53 Inhibits Bmi-1-driven Self-Renewal and Defines Salivary Gland Cancer Stemness Article Snippet: .. UM-HMC-1,-3A,-3B (RRID:CVCL_Y473, RRID:CVCL_Y471, RRID:CVCL_Y472, respectively) cells (23) were cultured in ultralow attachment plates or flasks (Corning) in DMEM/F12 (Invitrogen) supplemented with 1% N2 Supplement (Invitrogen), 1% GlutaMAX (Invitrogen), 1% antibiotic–antimycotic (Millipore Sigma), 20 ng/mL rhEGF (R&D Systems), 20 ng/mL Article Title: Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8 Article Snippet: The hESC line H9 (female XX line) was purchased from WiCell, Inc Cells are propagated as described previously ( ; ). .. In brief, undifferentiated hESCs were expanded on irradiated MEFs at 37°C with 5% CO 2 in hESC medium (KnockOut DMEM [Invitrogen, 10829018] with 20% knockout serum replacement [Invitrogen, 10828028] plus 1 mM L -glutamine, 0.1 mM nonessential amino acids, and 8 ng/ml Article Title: Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8 Article Snippet: All reagents and resources are listed on an appendix attached separately. hESC culture and hPGCLC induction The hESC line H9 (female XX line) was purchased from WiCell, Inc Cells are propagated as described previously (Jung et al., 2017; Kee et al., 2009). .. In brief, undifferentiated hESCs were expanded on irradiated MEFs at 37°C with 5% CO2 in hESC medium (KnockOut DMEM [Invitrogen, 10829018] with 20% knockout serum replacement [Invitrogen, 10828028] plus 1 mM L- glutamine, 0.1 mM nonessential amino acids, and 8 ng/ml Article Title: Molecular and functional characterization of different BrainSphere models for use in neurotoxicity testing on microelectrode arrays Article Snippet: The cells were transferred onto a poly-L-ornithine (PLO, 100 μg/mL; #P4957, Sigma-Aldrich, USA)-laminin (15 μg/mL; #LN521, Biolamina, Sweden)-coated 6-well plate and 10 μM Y-27632 (#HB2297, Hello Bio, Great Britain) were added for the first 24 h. On days 12 and 17, hiNPCs were passaged with Accutase (#07920, Stemcell Technologies, Canada) and transferred to a new PLO-laminin-coated plate. .. From day 12, the hiNPCS were cultivated in neural progenitor medium based on GNEIB medium without SB-431542 and LDN-193189, supplemented with 20 ng/mL Article Title: Molecular and Functional Characterization of Different BrainSphere Models for Use in Neurotoxicity Testing on Microelectrode Arrays. Article Snippet: On days 12 and 17, hiNPCs were passaged by enzymatic dissociation with Accutase (#07920, Stemcell Technologies, Vancouver, BC, Canada) for 10 min at 37 ◦C and 5% CO2 and transferred to a new PEI-laminin-coated 6-well plate. .. From day 12, the cells were cultivated in neural progenitor medium based on 2D-NIM medium without Cells 2023, 12, 1270 4 of 37 the dual SMAD inhibitors SB-431542 and LDN-193189 and supplemented with 20 ng/mL Article Title: Molecular and Functional Characterization of Different BrainSphere Models for Use in Neurotoxicity Testing on Microelectrode Arrays. Article Snippet: The cells were transferred onto a poly-L-ornithine (PLO, 100 μg/mL; #P4957, Sigma-Aldrich, St. Louis, MO, USA)laminin (15 μg/mL; #LN521, Biolamina, Sundbyberg, Sweden)-coated 6-well plate and 10 μM Y-27632 (#HB2297, Hello Bio, Bristol, UK) were added for the first 24 h. On days 12 and 17, hiNPCs were passaged with Accutase (#07920, Stemcell Technologies, Vancouver, BC, Canada) and transferred to a new PLO-laminin-coated plate. .. From day 12, the hiNPCS were cultivated in neural progenitor medium based on GNEIB medium without SB-431542 and LDN-193189, supplemented with 20 ng/mL Article Title: Early-Stage Quality - Late-Stage Confidence: Neural Induction Quality Control as a Key to Reproducible MEA-based Neurotoxicity Assays. Article Snippet: Human induced pluripotent stem cell (hiPSC)-derived neural models combined with microelectrode array (MEA)-based readouts are increasingly used in next-generation neurotoxicity assessment.. However, neural induction of hiPSCs into multipotent neural progenitor cells (hiNPCs) remains highly variable, and current quality control (QC) efforts focus largely on the pluripotent starting material.. As a result, failed neural inductions are often recognized only after weeks of differentiation during functional network analysis, causing substantial resource loss. Article Title: Molecular and functional characterization of different BrainSphere models for use in neurotoxicity testing on microelectrode arrays Article Snippet: On days 12 and 17, hiNPCs were passaged by enzymatic dissociation with Accutase (#07920, Stemcell Technologies, Canada) for 10 min at 37 °C and 5 % CO 2 and transferred to a new PEI-laminin-coated 6-well plate. .. From day 12, the cells were cultivated in neural progenitor medium based on 2D-NIM medium without the dual SMAD inhibitors SB-431542 and LDN-193189 and supplemented with 20 ng/mL Irradiation:Article Title: Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8 Article Snippet: The hESC line H9 (female XX line) was purchased from WiCell, Inc Cells are propagated as described previously ( ; ). .. In brief, undifferentiated hESCs were expanded on irradiated MEFs at 37°C with 5% CO 2 in hESC medium (KnockOut DMEM [Invitrogen, 10829018] with 20% knockout serum replacement [Invitrogen, 10828028] plus 1 mM L -glutamine, 0.1 mM nonessential amino acids, and 8 ng/ml Article Title: Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8 Article Snippet: All reagents and resources are listed on an appendix attached separately. hESC culture and hPGCLC induction The hESC line H9 (female XX line) was purchased from WiCell, Inc Cells are propagated as described previously (Jung et al., 2017; Kee et al., 2009). .. In brief, undifferentiated hESCs were expanded on irradiated MEFs at 37°C with 5% CO2 in hESC medium (KnockOut DMEM [Invitrogen, 10829018] with 20% knockout serum replacement [Invitrogen, 10828028] plus 1 mM L- glutamine, 0.1 mM nonessential amino acids, and 8 ng/ml Knock-Out:Article Title: Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8 Article Snippet: The hESC line H9 (female XX line) was purchased from WiCell, Inc Cells are propagated as described previously ( ; ). .. In brief, undifferentiated hESCs were expanded on irradiated MEFs at 37°C with 5% CO 2 in hESC medium (KnockOut DMEM [Invitrogen, 10829018] with 20% knockout serum replacement [Invitrogen, 10828028] plus 1 mM L -glutamine, 0.1 mM nonessential amino acids, and 8 ng/ml Article Title: Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8 Article Snippet: All reagents and resources are listed on an appendix attached separately. hESC culture and hPGCLC induction The hESC line H9 (female XX line) was purchased from WiCell, Inc Cells are propagated as described previously (Jung et al., 2017; Kee et al., 2009). .. In brief, undifferentiated hESCs were expanded on irradiated MEFs at 37°C with 5% CO2 in hESC medium (KnockOut DMEM [Invitrogen, 10829018] with 20% knockout serum replacement [Invitrogen, 10828028] plus 1 mM L- glutamine, 0.1 mM nonessential amino acids, and 8 ng/ml Passaging:Article Title: Molecular and functional characterization of different BrainSphere models for use in neurotoxicity testing on microelectrode arrays Article Snippet: The cells were transferred onto a poly-L-ornithine (PLO, 100 μg/mL; #P4957, Sigma-Aldrich, USA)-laminin (15 μg/mL; #LN521, Biolamina, Sweden)-coated 6-well plate and 10 μM Y-27632 (#HB2297, Hello Bio, Great Britain) were added for the first 24 h. On days 12 and 17, hiNPCs were passaged with Accutase (#07920, Stemcell Technologies, Canada) and transferred to a new PLO-laminin-coated plate. .. From day 12, the hiNPCS were cultivated in neural progenitor medium based on GNEIB medium without SB-431542 and LDN-193189, supplemented with 20 ng/mL Article Title: Molecular and Functional Characterization of Different BrainSphere Models for Use in Neurotoxicity Testing on Microelectrode Arrays. Article Snippet: On days 12 and 17, hiNPCs were passaged by enzymatic dissociation with Accutase (#07920, Stemcell Technologies, Vancouver, BC, Canada) for 10 min at 37 ◦C and 5% CO2 and transferred to a new PEI-laminin-coated 6-well plate. .. From day 12, the cells were cultivated in neural progenitor medium based on 2D-NIM medium without Cells 2023, 12, 1270 4 of 37 the dual SMAD inhibitors SB-431542 and LDN-193189 and supplemented with 20 ng/mL Article Title: Molecular and Functional Characterization of Different BrainSphere Models for Use in Neurotoxicity Testing on Microelectrode Arrays. Article Snippet: The cells were transferred onto a poly-L-ornithine (PLO, 100 μg/mL; #P4957, Sigma-Aldrich, St. Louis, MO, USA)laminin (15 μg/mL; #LN521, Biolamina, Sundbyberg, Sweden)-coated 6-well plate and 10 μM Y-27632 (#HB2297, Hello Bio, Bristol, UK) were added for the first 24 h. On days 12 and 17, hiNPCs were passaged with Accutase (#07920, Stemcell Technologies, Vancouver, BC, Canada) and transferred to a new PLO-laminin-coated plate. .. From day 12, the hiNPCS were cultivated in neural progenitor medium based on GNEIB medium without SB-431542 and LDN-193189, supplemented with 20 ng/mL Article Title: Molecular and functional characterization of different BrainSphere models for use in neurotoxicity testing on microelectrode arrays Article Snippet: On days 12 and 17, hiNPCs were passaged by enzymatic dissociation with Accutase (#07920, Stemcell Technologies, Canada) for 10 min at 37 °C and 5 % CO 2 and transferred to a new PEI-laminin-coated 6-well plate. .. From day 12, the cells were cultivated in neural progenitor medium based on 2D-NIM medium without the dual SMAD inhibitors SB-431542 and LDN-193189 and supplemented with 20 ng/mL Centrifugation:Article Title: Early-Stage Quality - Late-Stage Confidence: Neural Induction Quality Control as a Key to Reproducible MEA-based Neurotoxicity Assays. Article Snippet: Human induced pluripotent stem cell (hiPSC)-derived neural models combined with microelectrode array (MEA)-based readouts are increasingly used in next-generation neurotoxicity assessment.. However, neural induction of hiPSCs into multipotent neural progenitor cells (hiNPCs) remains highly variable, and current quality control (QC) efforts focus largely on the pluripotent starting material.. As a result, failed neural inductions are often recognized only after weeks of differentiation during functional network analysis, causing substantial resource loss. |